This study was designed to investigate the clinico-haemato-biochemical, rumen fl uid and peritoneal fl uid alteration in buffaloes

with peritonitis. Buffaloes with peritonitis had anorexia, dehydration, abdominal distension, ruminal atony, pain, fever, reduced milk yield and loss of defecation. Pungent smelling peritoneal fluid was yielded on abdominocentesis. Hemato-biochemical alterations revealed absolute neutrophilia, increased levels of plasma aspartate aminotransferase, alkaline phosphatase, bilirubin, glucose, blood urea nitrogen, creatinine, globulin and fi brinogen and decreased levels of plasma sodium, potassium, calcium and chloride. Peritoneal fl uid analysis showed increased total leucocyte count, neutrophil count and total protein level. Whereas, rumen liquor of diseased buffaloes had microbial inactivity, increased methylene blue reduction time, increased ammonia nitrogen and decreased total volatile fatty acids.